ARIAKE KURO
| Usefulness | Pond improvement agent for aquaculture |
| Registration number | KKP RI No. D 2112564 PbBP |
| Active ingredients | Bacillus amyloliquefaciens strain D2018 Bacillus subtilis strain D2558 |
| Characteristics | Charcoal tablets |
| Packing | 500 g/bottle |
Features
- Charcoal tablets with two bacteria Bacillus amyloliquefaciens D2018 and Bacillus subtilis D2558 isolated from marine enviroment.
- ARIAKE KURO has the following activity in aerob and anaerobe conditions. - to break down various of organic matter. - to reduce Nitrite (NO2) and Nitrate (NO3).
- Active ingredients are made in Japan.
Bacteria
Bacillus subtilis D2558
This strain was isolated from the intestine of wild Kuruma shrimp. D2558 is a facultative anaerobic bacterium.
Bacillus amyloliquefaciens D2018
This strain was isolated from the intestine of wild Kuruma shrimp. D2018 is a facultative anaerobic bacterium.
Dose (5,000 m2)
| Stages / Conditions | Dose |
|---|---|
| Preparation | Spread 1 kg evenly (mud area) while drying stage |
| Maintenance | DOC ≥ 40, 250 gram after siphon
or mud accumulate (dead zone and central drain) in the bottom pond every 3 days |
| NH4, NO2, and NO3 treatment | 500 grams every 2 days |
| Heavy pollution | Spread 1-5 tablets / m2 (mud area) |
Mode of Action
- Reduction of Nitrite (NO2) and Nitrate acid (NO3)
- Degradation of various organic matters
Experiment Data
(1) Reduction Nitrogen
0.1 gram of ARIAKE KURO was incubated under aerobic or anaerobic condition, at 27℃ in 10 ml test liquid media added ammonia, nitrous acid or nitric acid. Quantitative of nitrogen compounds were measured after 1, 2, 3, 7, 10, 14, 21 days by HPLC analysis.
(2) Degradation of Organic Matters
Degradation test against organic matters was determined by the agar plate assay for proteolytic activity on skim milk, amylolytic activity on starch. Two strains in ARIAKE KURO were inoculated on each organic matter plates. The clear zone were formed around the areas where the strain had degrade the target-organic matter.
Degradation capability against organic matters by strains in ARIAKE KURO under anaerobic condition.
Safety Precautions
- Store in a cool, dry place, avoid direct sunlight.- Please use in quality assurance period (2 years after month of manufacture).
